Describe the Reagents Used and Purpose of the Gram Stain
Crystal Violet CV is the Primary stain and is used to color both Gram-positive and Gram-negative bacteria purple. A primary color and a counterstain. Solved 2 What Are The Four Reagents Of A Gram Stain And Chegg Com Slightly tilt slide and rinse gently with tap water or distilled water using a wash bottle. . Gram-negative bacteria are decolorized by the alcohol losing the color of the primary stain purple. Gram-staining is a test to classify the bacteria. Gram-positive cells form a crystal violet-iodine complex. After decolorization step a. Ethanol Safranin secondary stain Water preferably in a squirt bottle Make a slide of cell sample to be stained. Why is it important to use fresh. It is added to chemically change the shape of the dye molecule and therefore trap it in the cell wall. Let it stand for one minute. Explain the process for a Gram stain Describe what each of the reagents is used. ...